Piezo-actuated Mouse ICSI (Intracytoplasmic sperm injection)
نویسنده
چکیده
Intracytoplasmic sperm injection (ICSI) is not only used in human assisted reproduction (ART), but it is also widely employed for veterinary in vitro fertilization during rare species preservation, germ-line rescue of transgenic animals or for any veterinary assisted conception. Piezo-assisted ICSI, which was first described in 1995 [1], can be employed for assisted conception in animals in which standard ICSI fails, such as mice. The microinjection workstation required for this technique is very similar to standard ICSI, but with the addition of a piezo impact unit attached to the capillary holder. This Userguide focuses on the piezo-assisted microinjection procedure itself. Abstract
منابع مشابه
Comparison of Embryo Development between Intracytoplasmic and Piezo-Assisted Sperm Injection after Treating Mouse Sperms by Ca2+ Ionophore
The purpose of this study was to evaluate the efficiency of intracytoplasmic sperm injection (ICSI) and Piezo-assisted sperm injection after pretreatment with calcium ionophore (CaI) on the mouse embryo development. Methods: In this study, the conventional ICSI and Piezo-ICSI procedures were used. The efficacy of the methods was examined after mouse matured oocytes were fertilized with or witho...
متن کاملComparison of Embryo Development between Intracytoplasmic and Piezo-Assisted Sperm Injection after Treating Mouse Sperms by Ca Ionophore
Background: The purpose of this study was to evaluate the efficiency of intracytoplasmic sperm injection (ICSI) and Piezo-assisted sperm injection after pretreatment with calcium ionophore (CaI) on the mouse embryo development. Methods: In this study, the conventional ICSI and Piezo-ICSI procedures were used. The efficacy of the methods was examined after mouse matured oocytes were fertilized w...
متن کاملComparison of embryo development between intracytoplasmic and Piezo-assisted sperm injection after treating mouse sperms by Ca2+ ionophore.
BACKGROUND The purpose of this study was to evaluate the efficiency of intracytoplasmic sperm injection (ICSI) and Piezo-assisted sperm injection after pretreatment with calcium ionophore (CaI) on the mouse embryo development. METHODS In this study, the conventional ICSI and Piezo-ICSI procedures were used. The efficacy of the methods was examined after mouse matured oocytes were fertilized w...
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In human fertilization, the sperm centrosome nucleates a radial array of microtubules called the sperm aster. The sperm aster is responsible for apposition of male and female pronuclei, and later gives rise to the first meiotic spindle. The objective of this study was to determine microtubule assembly and chromatin configuration in rabbit oocytes following intracytoplasmic injection with human ...
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Conventionally, intracytoplasmic sperm injection (ICSI) in the mouse is conducted with piezo-drills that use a droplet of mercury for damping. The use of mercury causes concerns of toxicity and contamination. Although Fluorinert can be used as a substitute for mercury to reduce piezo-drill’s lateral vibration, the damping effect is not as satisfactory as mercury. In this work, a modified piezo-...
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